Penicillin Acylase Activity of Penicillium chrysogenum
نویسندگان
چکیده
منابع مشابه
Penicillin acyltransferase in Penicillium chrysogenum.
Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase was purified threefold by adsorption on calcium phosphate gel at pH 6 and elutio...
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A great deal of work has been directed toward increasing the yield of penicillin in fermentations with Penicillium chrysogenum. The spectacular increase in yield which has been achieved has resulted, in the main, from two approaches. In the first approach, mutant strains of P. chrysogenum produced by spontaneous variation or by the action of mutagenic agents such as nitrogen mustard or ultravio...
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The present study was aimed at exploring the native wild isolates of Penicillium chrysogenum series in terms of their penicillin production potential. Apart from the standard medium, the efforts were made to utilize suitable agro-industrial wastes for the maximum yield of penicillin. Two series of P. chrysogenum were isolated from local sources and named as P. chrysogenum series UAF R1 and P. c...
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Sampling and storage. The fermentations were conducted in the 100-gallon tanks described by Stefaniak et al. (1946). Samples were removed periodically into 500-ml flasks and ifitered. A 0.5-ml filtered sample was immediately diluted for penicillin assay by addition to previously chilled phosphate buffer in volumetric flasks (4.0 g KH2P04 and 1.0 g K2HPO4 per 500 ml solution in distilled water; ...
متن کاملPenicillin production by high-yielding strains of Penicillium chrysogenum.
The development and study of strains of Penicillium chrysogenum that give high yields of penicillin has been a major project at the University of Wisconsin during the past 12 years. Many thousands of cultures have been screened for their penicillin-producing ability in the Botany Department of the University. The methods used for the production and screening of mutants, the genealogy of the out...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1965
ISSN: 0003-6919
DOI: 10.1128/am.13.5.738-742.1965